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Manuscript N A Shscr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Blocking Assay:Article Title: miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GW843682X MCE HY-11003 Minoxidil MCE HY-B0112 DAPI Thermo 62247 HelixNP NIR Biolegend 425301 Tris/glycine/SDS buffer Epizyme PS105S Fast transfer buffer Epizyme PS117 Protein sample loading buffer Epizyme LT101S Protein free rapid blocking buffer Epizyme PS108 Primary antibody dilution buffer for WB Epizyme PS114 Secondary antibody dilution buffer for WB Epizyme PS115 Protein marker Beyotime Biotech P0068 Critical commercial assays Click-iT EdU alexa aluor 488 Flow cytometry assay Life C10632 BeyoClick EdU cell proliferation kit with alexa fluor 647 Beyotime Biotech C0081S PrimeScriptRT reagent kit TAKARA RR047B Hairpin-itTM microRNA and U6 snRNA normalization RT-PCR quantitation kit GenePharma E22007 SDS-PAGE gel quick preparation kit) Beyotime P0012AC Mir-X miRNA first-strand synthesis kit TAKARA 638315 DIG oligonucleotide 30 end labeling kit Roche 03353575910 Secrete-pairTM dual luminescence assay kit GeneCopoeia LF031 Femto light chemiluminescence kit Epizyme SQ201 Deposited data RNaseq data this manuscript https://www.biosino.org/node/project/ detail/OEP001189 Experimental models: cell lines Primary mouse keratinocytes (MK) this manuscript N/A 293FT Invitrogen R70007 Experimental models: organisms/strains Mouse: K14-Cre Gift from Dr. Jun Qin lab (Dassule et al., 2000) N/A Mouse: K14-rtTA Gift from Dr. Elaine Fuchs lab (Nguyen et al., 2006; Zhang et al., 2011) N/A Mouse: TRE-miR-24 this manuscript N/A Mouse: Mir24-1fl/fl this manuscript N/A Mouse: Mir24-2ko/ko this manuscript N/A Oligonucleotides See Table S1 for oligonucleotide information N/A Recombinant DNA pTRE2-miR24 this manuscript N/A PLVX-Tetone-puro YouBio VT9002 pLKO.1-puro Addgene 10878 pCDH-CMV-MCS-EF1-Puro SBI CD510B pmiRFP709-N1 Western Blot:Article Title: miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GW843682X MCE HY-11003 Minoxidil MCE HY-B0112 DAPI Thermo 62247 HelixNP NIR Biolegend 425301 Tris/glycine/SDS buffer Epizyme PS105S Fast transfer buffer Epizyme PS117 Protein sample loading buffer Epizyme LT101S Protein free rapid blocking buffer Epizyme PS108 Primary antibody dilution buffer for WB Epizyme PS114 Secondary antibody dilution buffer for WB Epizyme PS115 Protein marker Beyotime Biotech P0068 Critical commercial assays Click-iT EdU alexa aluor 488 Flow cytometry assay Life C10632 BeyoClick EdU cell proliferation kit with alexa fluor 647 Beyotime Biotech C0081S PrimeScriptRT reagent kit TAKARA RR047B Hairpin-itTM microRNA and U6 snRNA normalization RT-PCR quantitation kit GenePharma E22007 SDS-PAGE gel quick preparation kit) Beyotime P0012AC Mir-X miRNA first-strand synthesis kit TAKARA 638315 DIG oligonucleotide 30 end labeling kit Roche 03353575910 Secrete-pairTM dual luminescence assay kit GeneCopoeia LF031 Femto light chemiluminescence kit Epizyme SQ201 Deposited data RNaseq data this manuscript https://www.biosino.org/node/project/ detail/OEP001189 Experimental models: cell lines Primary mouse keratinocytes (MK) this manuscript N/A 293FT Invitrogen R70007 Experimental models: organisms/strains Mouse: K14-Cre Gift from Dr. Jun Qin lab (Dassule et al., 2000) N/A Mouse: K14-rtTA Gift from Dr. Elaine Fuchs lab (Nguyen et al., 2006; Zhang et al., 2011) N/A Mouse: TRE-miR-24 this manuscript N/A Mouse: Mir24-1fl/fl this manuscript N/A Mouse: Mir24-2ko/ko this manuscript N/A Oligonucleotides See Table S1 for oligonucleotide information N/A Recombinant DNA pTRE2-miR24 this manuscript N/A PLVX-Tetone-puro YouBio VT9002 pLKO.1-puro Addgene 10878 pCDH-CMV-MCS-EF1-Puro SBI CD510B pmiRFP709-N1 Marker:Article Title: miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GW843682X MCE HY-11003 Minoxidil MCE HY-B0112 DAPI Thermo 62247 HelixNP NIR Biolegend 425301 Tris/glycine/SDS buffer Epizyme PS105S Fast transfer buffer Epizyme PS117 Protein sample loading buffer Epizyme LT101S Protein free rapid blocking buffer Epizyme PS108 Primary antibody dilution buffer for WB Epizyme PS114 Secondary antibody dilution buffer for WB Epizyme PS115 Protein marker Beyotime Biotech P0068 Critical commercial assays Click-iT EdU alexa aluor 488 Flow cytometry assay Life C10632 BeyoClick EdU cell proliferation kit with alexa fluor 647 Beyotime Biotech C0081S PrimeScriptRT reagent kit TAKARA RR047B Hairpin-itTM microRNA and U6 snRNA normalization RT-PCR quantitation kit GenePharma E22007 SDS-PAGE gel quick preparation kit) Beyotime P0012AC Mir-X miRNA first-strand synthesis kit TAKARA 638315 DIG oligonucleotide 30 end labeling kit Roche 03353575910 Secrete-pairTM dual luminescence assay kit GeneCopoeia LF031 Femto light chemiluminescence kit Epizyme SQ201 Deposited data RNaseq data this manuscript https://www.biosino.org/node/project/ detail/OEP001189 Experimental models: cell lines Primary mouse keratinocytes (MK) this manuscript N/A 293FT Invitrogen R70007 Experimental models: organisms/strains Mouse: K14-Cre Gift from Dr. Jun Qin lab (Dassule et al., 2000) N/A Mouse: K14-rtTA Gift from Dr. Elaine Fuchs lab (Nguyen et al., 2006; Zhang et al., 2011) N/A Mouse: TRE-miR-24 this manuscript N/A Mouse: Mir24-1fl/fl this manuscript N/A Mouse: Mir24-2ko/ko this manuscript N/A Oligonucleotides See Table S1 for oligonucleotide information N/A Recombinant DNA pTRE2-miR24 this manuscript N/A PLVX-Tetone-puro YouBio VT9002 pLKO.1-puro Addgene 10878 pCDH-CMV-MCS-EF1-Puro SBI CD510B pmiRFP709-N1 Flow Cytometry:Article Title: miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GW843682X MCE HY-11003 Minoxidil MCE HY-B0112 DAPI Thermo 62247 HelixNP NIR Biolegend 425301 Tris/glycine/SDS buffer Epizyme PS105S Fast transfer buffer Epizyme PS117 Protein sample loading buffer Epizyme LT101S Protein free rapid blocking buffer Epizyme PS108 Primary antibody dilution buffer for WB Epizyme PS114 Secondary antibody dilution buffer for WB Epizyme PS115 Protein marker Beyotime Biotech P0068 Critical commercial assays Click-iT EdU alexa aluor 488 Flow cytometry assay Life C10632 BeyoClick EdU cell proliferation kit with alexa fluor 647 Beyotime Biotech C0081S PrimeScriptRT reagent kit TAKARA RR047B Hairpin-itTM microRNA and U6 snRNA normalization RT-PCR quantitation kit GenePharma E22007 SDS-PAGE gel quick preparation kit) Beyotime P0012AC Mir-X miRNA first-strand synthesis kit TAKARA 638315 DIG oligonucleotide 30 end labeling kit Roche 03353575910 Secrete-pairTM dual luminescence assay kit GeneCopoeia LF031 Femto light chemiluminescence kit Epizyme SQ201 Deposited data RNaseq data this manuscript https://www.biosino.org/node/project/ detail/OEP001189 Experimental models: cell lines Primary mouse keratinocytes (MK) this manuscript N/A 293FT Invitrogen R70007 Experimental models: organisms/strains Mouse: K14-Cre Gift from Dr. Jun Qin lab (Dassule et al., 2000) N/A Mouse: K14-rtTA Gift from Dr. Elaine Fuchs lab (Nguyen et al., 2006; Zhang et al., 2011) N/A Mouse: TRE-miR-24 this manuscript N/A Mouse: Mir24-1fl/fl this manuscript N/A Mouse: Mir24-2ko/ko this manuscript N/A Oligonucleotides See Table S1 for oligonucleotide information N/A Recombinant DNA pTRE2-miR24 this manuscript N/A PLVX-Tetone-puro YouBio VT9002 pLKO.1-puro Addgene 10878 pCDH-CMV-MCS-EF1-Puro SBI CD510B pmiRFP709-N1 Reverse Transcription Polymerase Chain Reaction:Article Title: miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GW843682X MCE HY-11003 Minoxidil MCE HY-B0112 DAPI Thermo 62247 HelixNP NIR Biolegend 425301 Tris/glycine/SDS buffer Epizyme PS105S Fast transfer buffer Epizyme PS117 Protein sample loading buffer Epizyme LT101S Protein free rapid blocking buffer Epizyme PS108 Primary antibody dilution buffer for WB Epizyme PS114 Secondary antibody dilution buffer for WB Epizyme PS115 Protein marker Beyotime Biotech P0068 Critical commercial assays Click-iT EdU alexa aluor 488 Flow cytometry assay Life C10632 BeyoClick EdU cell proliferation kit with alexa fluor 647 Beyotime Biotech C0081S PrimeScriptRT reagent kit TAKARA RR047B Hairpin-itTM microRNA and U6 snRNA normalization RT-PCR quantitation kit GenePharma E22007 SDS-PAGE gel quick preparation kit) Beyotime P0012AC Mir-X miRNA first-strand synthesis kit TAKARA 638315 DIG oligonucleotide 30 end labeling kit Roche 03353575910 Secrete-pairTM dual luminescence assay kit GeneCopoeia LF031 Femto light chemiluminescence kit Epizyme SQ201 Deposited data RNaseq data this manuscript https://www.biosino.org/node/project/ detail/OEP001189 Experimental models: cell lines Primary mouse keratinocytes (MK) this manuscript N/A 293FT Invitrogen R70007 Experimental models: organisms/strains Mouse: K14-Cre Gift from Dr. Jun Qin lab (Dassule et al., 2000) N/A Mouse: K14-rtTA Gift from Dr. Elaine Fuchs lab (Nguyen et al., 2006; Zhang et al., 2011) N/A Mouse: TRE-miR-24 this manuscript N/A Mouse: Mir24-1fl/fl this manuscript N/A Mouse: Mir24-2ko/ko this manuscript N/A Oligonucleotides See Table S1 for oligonucleotide information N/A Recombinant DNA pTRE2-miR24 this manuscript N/A PLVX-Tetone-puro YouBio VT9002 pLKO.1-puro Addgene 10878 pCDH-CMV-MCS-EF1-Puro SBI CD510B pmiRFP709-N1 Quantitation Assay:Article Title: miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GW843682X MCE HY-11003 Minoxidil MCE HY-B0112 DAPI Thermo 62247 HelixNP NIR Biolegend 425301 Tris/glycine/SDS buffer Epizyme PS105S Fast transfer buffer Epizyme PS117 Protein sample loading buffer Epizyme LT101S Protein free rapid blocking buffer Epizyme PS108 Primary antibody dilution buffer for WB Epizyme PS114 Secondary antibody dilution buffer for WB Epizyme PS115 Protein marker Beyotime Biotech P0068 Critical commercial assays Click-iT EdU alexa aluor 488 Flow cytometry assay Life C10632 BeyoClick EdU cell proliferation kit with alexa fluor 647 Beyotime Biotech C0081S PrimeScriptRT reagent kit TAKARA RR047B Hairpin-itTM microRNA and U6 snRNA normalization RT-PCR quantitation kit GenePharma E22007 SDS-PAGE gel quick preparation kit) Beyotime P0012AC Mir-X miRNA first-strand synthesis kit TAKARA 638315 DIG oligonucleotide 30 end labeling kit Roche 03353575910 Secrete-pairTM dual luminescence assay kit GeneCopoeia LF031 Femto light chemiluminescence kit Epizyme SQ201 Deposited data RNaseq data this manuscript https://www.biosino.org/node/project/ detail/OEP001189 Experimental models: cell lines Primary mouse keratinocytes (MK) this manuscript N/A 293FT Invitrogen R70007 Experimental models: organisms/strains Mouse: K14-Cre Gift from Dr. Jun Qin lab (Dassule et al., 2000) N/A Mouse: K14-rtTA Gift from Dr. Elaine Fuchs lab (Nguyen et al., 2006; Zhang et al., 2011) N/A Mouse: TRE-miR-24 this manuscript N/A Mouse: Mir24-1fl/fl this manuscript N/A Mouse: Mir24-2ko/ko this manuscript N/A Oligonucleotides See Table S1 for oligonucleotide information N/A Recombinant DNA pTRE2-miR24 this manuscript N/A PLVX-Tetone-puro YouBio VT9002 pLKO.1-puro Addgene 10878 pCDH-CMV-MCS-EF1-Puro SBI CD510B pmiRFP709-N1 SDS Page:Article Title: miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GW843682X MCE HY-11003 Minoxidil MCE HY-B0112 DAPI Thermo 62247 HelixNP NIR Biolegend 425301 Tris/glycine/SDS buffer Epizyme PS105S Fast transfer buffer Epizyme PS117 Protein sample loading buffer Epizyme LT101S Protein free rapid blocking buffer Epizyme PS108 Primary antibody dilution buffer for WB Epizyme PS114 Secondary antibody dilution buffer for WB Epizyme PS115 Protein marker Beyotime Biotech P0068 Critical commercial assays Click-iT EdU alexa aluor 488 Flow cytometry assay Life C10632 BeyoClick EdU cell proliferation kit with alexa fluor 647 Beyotime Biotech C0081S PrimeScriptRT reagent kit TAKARA RR047B Hairpin-itTM microRNA and U6 snRNA normalization RT-PCR quantitation kit GenePharma E22007 SDS-PAGE gel quick preparation kit) Beyotime P0012AC Mir-X miRNA first-strand synthesis kit TAKARA 638315 DIG oligonucleotide 30 end labeling kit Roche 03353575910 Secrete-pairTM dual luminescence assay kit GeneCopoeia LF031 Femto light chemiluminescence kit Epizyme SQ201 Deposited data RNaseq data this manuscript https://www.biosino.org/node/project/ detail/OEP001189 Experimental models: cell lines Primary mouse keratinocytes (MK) this manuscript N/A 293FT Invitrogen R70007 Experimental models: organisms/strains Mouse: K14-Cre Gift from Dr. Jun Qin lab (Dassule et al., 2000) N/A Mouse: K14-rtTA Gift from Dr. Elaine Fuchs lab (Nguyen et al., 2006; Zhang et al., 2011) N/A Mouse: TRE-miR-24 this manuscript N/A Mouse: Mir24-1fl/fl this manuscript N/A Mouse: Mir24-2ko/ko this manuscript N/A Oligonucleotides See Table S1 for oligonucleotide information N/A Recombinant DNA pTRE2-miR24 this manuscript N/A PLVX-Tetone-puro YouBio VT9002 pLKO.1-puro Addgene 10878 pCDH-CMV-MCS-EF1-Puro SBI CD510B pmiRFP709-N1 End Labeling:Article Title: miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GW843682X MCE HY-11003 Minoxidil MCE HY-B0112 DAPI Thermo 62247 HelixNP NIR Biolegend 425301 Tris/glycine/SDS buffer Epizyme PS105S Fast transfer buffer Epizyme PS117 Protein sample loading buffer Epizyme LT101S Protein free rapid blocking buffer Epizyme PS108 Primary antibody dilution buffer for WB Epizyme PS114 Secondary antibody dilution buffer for WB Epizyme PS115 Protein marker Beyotime Biotech P0068 Critical commercial assays Click-iT EdU alexa aluor 488 Flow cytometry assay Life C10632 BeyoClick EdU cell proliferation kit with alexa fluor 647 Beyotime Biotech C0081S PrimeScriptRT reagent kit TAKARA RR047B Hairpin-itTM microRNA and U6 snRNA normalization RT-PCR quantitation kit GenePharma E22007 SDS-PAGE gel quick preparation kit) Beyotime P0012AC Mir-X miRNA first-strand synthesis kit TAKARA 638315 DIG oligonucleotide 30 end labeling kit Roche 03353575910 Secrete-pairTM dual luminescence assay kit GeneCopoeia LF031 Femto light chemiluminescence kit Epizyme SQ201 Deposited data RNaseq data this manuscript https://www.biosino.org/node/project/ detail/OEP001189 Experimental models: cell lines Primary mouse keratinocytes (MK) this manuscript N/A 293FT Invitrogen R70007 Experimental models: organisms/strains Mouse: K14-Cre Gift from Dr. Jun Qin lab (Dassule et al., 2000) N/A Mouse: K14-rtTA Gift from Dr. Elaine Fuchs lab (Nguyen et al., 2006; Zhang et al., 2011) N/A Mouse: TRE-miR-24 this manuscript N/A Mouse: Mir24-1fl/fl this manuscript N/A Mouse: Mir24-2ko/ko this manuscript N/A Oligonucleotides See Table S1 for oligonucleotide information N/A Recombinant DNA pTRE2-miR24 this manuscript N/A PLVX-Tetone-puro YouBio VT9002 pLKO.1-puro Addgene 10878 pCDH-CMV-MCS-EF1-Puro SBI CD510B pmiRFP709-N1 Luminescence Assay:Article Title: miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GW843682X MCE HY-11003 Minoxidil MCE HY-B0112 DAPI Thermo 62247 HelixNP NIR Biolegend 425301 Tris/glycine/SDS buffer Epizyme PS105S Fast transfer buffer Epizyme PS117 Protein sample loading buffer Epizyme LT101S Protein free rapid blocking buffer Epizyme PS108 Primary antibody dilution buffer for WB Epizyme PS114 Secondary antibody dilution buffer for WB Epizyme PS115 Protein marker Beyotime Biotech P0068 Critical commercial assays Click-iT EdU alexa aluor 488 Flow cytometry assay Life C10632 BeyoClick EdU cell proliferation kit with alexa fluor 647 Beyotime Biotech C0081S PrimeScriptRT reagent kit TAKARA RR047B Hairpin-itTM microRNA and U6 snRNA normalization RT-PCR quantitation kit GenePharma E22007 SDS-PAGE gel quick preparation kit) Beyotime P0012AC Mir-X miRNA first-strand synthesis kit TAKARA 638315 DIG oligonucleotide 30 end labeling kit Roche 03353575910 Secrete-pairTM dual luminescence assay kit GeneCopoeia LF031 Femto light chemiluminescence kit Epizyme SQ201 Deposited data RNaseq data this manuscript https://www.biosino.org/node/project/ detail/OEP001189 Experimental models: cell lines Primary mouse keratinocytes (MK) this manuscript N/A 293FT Invitrogen R70007 Experimental models: organisms/strains Mouse: K14-Cre Gift from Dr. Jun Qin lab (Dassule et al., 2000) N/A Mouse: K14-rtTA Gift from Dr. Elaine Fuchs lab (Nguyen et al., 2006; Zhang et al., 2011) N/A Mouse: TRE-miR-24 this manuscript N/A Mouse: Mir24-1fl/fl this manuscript N/A Mouse: Mir24-2ko/ko this manuscript N/A Oligonucleotides See Table S1 for oligonucleotide information N/A Recombinant DNA pTRE2-miR24 this manuscript N/A PLVX-Tetone-puro YouBio VT9002 pLKO.1-puro Addgene 10878 pCDH-CMV-MCS-EF1-Puro SBI CD510B pmiRFP709-N1 Recombinant:Article Title: miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GW843682X MCE HY-11003 Minoxidil MCE HY-B0112 DAPI Thermo 62247 HelixNP NIR Biolegend 425301 Tris/glycine/SDS buffer Epizyme PS105S Fast transfer buffer Epizyme PS117 Protein sample loading buffer Epizyme LT101S Protein free rapid blocking buffer Epizyme PS108 Primary antibody dilution buffer for WB Epizyme PS114 Secondary antibody dilution buffer for WB Epizyme PS115 Protein marker Beyotime Biotech P0068 Critical commercial assays Click-iT EdU alexa aluor 488 Flow cytometry assay Life C10632 BeyoClick EdU cell proliferation kit with alexa fluor 647 Beyotime Biotech C0081S PrimeScriptRT reagent kit TAKARA RR047B Hairpin-itTM microRNA and U6 snRNA normalization RT-PCR quantitation kit GenePharma E22007 SDS-PAGE gel quick preparation kit) Beyotime P0012AC Mir-X miRNA first-strand synthesis kit TAKARA 638315 DIG oligonucleotide 30 end labeling kit Roche 03353575910 Secrete-pairTM dual luminescence assay kit GeneCopoeia LF031 Femto light chemiluminescence kit Epizyme SQ201 Deposited data RNaseq data this manuscript https://www.biosino.org/node/project/ detail/OEP001189 Experimental models: cell lines Primary mouse keratinocytes (MK) this manuscript N/A 293FT Invitrogen R70007 Experimental models: organisms/strains Mouse: K14-Cre Gift from Dr. Jun Qin lab (Dassule et al., 2000) N/A Mouse: K14-rtTA Gift from Dr. Elaine Fuchs lab (Nguyen et al., 2006; Zhang et al., 2011) N/A Mouse: TRE-miR-24 this manuscript N/A Mouse: Mir24-1fl/fl this manuscript N/A Mouse: Mir24-2ko/ko this manuscript N/A Oligonucleotides See Table S1 for oligonucleotide information N/A Recombinant DNA pTRE2-miR24 this manuscript N/A PLVX-Tetone-puro YouBio VT9002 pLKO.1-puro Addgene 10878 pCDH-CMV-MCS-EF1-Puro SBI CD510B pmiRFP709-N1 |